Increased N-acetyl-β-glucosaminidase activity in primary breast carcinomas corresponds to a decrease in N-acetylglucosamine containing proteins

Is urinary N-acetyl-beta-D-glucosaminidase a marker of urological abnormality in children?

  Abstract   Background: Hydronephrosis is the most common congenital condition that is detected   by prenatal ultrasonography. Moreover, the widespread use of prenatal ultrasonography   results in an increased recognition of fetal hydronephrosis. Prenatal hydronephrosis   is diagnosed at an incidence of 1:100 to 1:500 by ultrasonographic studies. The presence of hydronephrosis is not synonymou...

Down-regulation of β-N-acetyl-D-glucosaminidase increases Akt1 activity in thyroid anaplastic cancer cells.

O-GlcNAcylation is a common and dynamic modification of intracellular proteins in which β-N-acetyl-glucosamine moieties are attached to hydroxyl groups of serine or threonine residues (O-GlcNAc). Accumulating evidence suggests the critical role of protein O-GlcNAcylation in signal transduction, transcriptional control, cell cycle regulation and protein degradation. However, the exact role of O-...

Studies on glucosaminidase; N-acetyl-beta-glucosaminidase in rat kidney.

Increased urinary N-acetyl-beta-D-glucosaminidase activity in children with hydronephrosis.

OBJECTIVE Hydronephrosis leads to deterioration of renal function. As urinary N-acetyl-beta-D-glucosaminidase (U-NAG) activity is considered a sensitive marker of renal tubular impairment, our aim was to measure U-NAG in children with hydronephrosis and to look for a relationship among selected clinical parameters. MATERIALS AND METHODS We studied 31 children (22 boys and 9 girls, mean age 2....

Quantification of Lysosomal Membrane Permeabilization by Cytosolic Cathepsin and β-N-Acetyl-Glucosaminidase Activity Measurements.

Programmed cell death involving lysosomal membrane permeabilization (LMP) is an alternative cell death pathway induced under various cellular conditions and by numerous cytotoxic stimuli. The method presented here to quantify LMP takes advantage of the detergent digitonin, which creates pores in cellular membranes by replacing cholesterol. The difference in cholesterol content between the plasm...